Study #2860: Role of microRNA on age and contraction-induced skeletal muscle growth

Purpose
This research study will compare how the activation of specific small RNA molecules (microRNA) in muscle differs in response to a single round of resistance exercise (RE) between young and older men and women.

Target Population
We are seeking healthy sedentary young individuals between the ages of 20-30 yrs. and sedentary older adults 70-90 yrs. who have noticed that they are having problems performing routine activities of daily living. Some examples of these may include having difficulty lifting and carrying objects, rising out of a chair, climbing a flight of stairs or walking a quarter of a mile or more without stopping to rest.

This research study will compare how the activation of specific small RNA molecules (microRNA) in muscle differs in response to a single round of resistance exercise (RE) between young and older men and women.

If you meet the eligibility criteria and are accepted into the study, you will be asked to:

  • Undergo 4 muscle biopsies and 3 blood draws
  • Undergo 1 strength assessment and 1 round of exercise
  • Optional overnight stay at the HNRCA’s Metabolic Research Unit

Major Exclusions
Lost or gained 7.5% or more of their body weight in the past 6 months
Acute or terminal disease
Significant immune disorder
Types I and II Diabetes mellitus
Uncontrolled hypertension (>180/100 mmHg)
Neuromuscular disease
Subjects must also not be participating in any regular endurance or resistance training exercise during the previous six months.
Upper or lower extremity fracture in the previous 6 months
Allergy to lidocaine
Anticoagulant therapy (Warfarin, Coumadin, or Plavix)
ACE inhibitors
Androgen or estrogen therapy or other hormone replacement therapy
Chronic corticosteroid therapy
BMI <19       or >35 kg/m2

Length of Study
2 weeks, including 3 study visits

Number of Visits
1 screening visit, 3 study visits for collection of blood, urine, 4 muscle biopsies and clinical information (i.e., blood pressure and weight) over a total of 3 weeks.

Residency
Optional overnight stay

Can I work?
Yes

Study diet
none

Stipend
$25 for screening, $20 for DXA scan and up to $475 for participants who qualify and complete the entire study.

For more information about this study please call 617-556-3082 or email MuscleExerciseLab@tufts.edu

NOTES:
Length of study

Total length of the study including resident stays and nonresident visits.
This does not mean you will be testing the entire time.
Residency

The length of time volunteers are required to stay overnight at the HNRCA. Residents have their own private room, bathroom, television, VCR, and phone during their stay.
Payment

The amount of reimbursement for travel and inconvenience.
Number of visits

Total number of nonresident visits.
Study diet

Indicates meals which will be provided by the HNRCA
Can I work?

Indicates if you will be able to work and if so, when.

For more information about this study please call 617-556-3082 or email MuscleExerciseLab@tufts.edu

We appreciate your interest in our program and we hope to work with you in the future.

Thank you,
The Recruitment Office
Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University